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antibodies against fap  (Bioss)


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    Bioss antibodies against fap
    Antibodies Against Fap, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibodies+against+fap/FAPA+Polyclonal+Antibody/pm41595198-70-34-39
    Average 94 stars, based on 22 article reviews
    antibodies against fap - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Modulation of Cancer-Associated Fibroblasts via the miR-624-5p/FAP Axis Drives Progression and Metastasis in Non-Small Cell Lung Cancer
    Article Snippet: .. Cells were fixed with 4% paraformaldehyde for 30 min, followed by PBS washing, permeabilization with 0.5% Triton X-100 (Solarbio, Beijing, China), and blocking with 10% BSA, and then incubated overnight at 4 °C with antibodies against FAP (Bs-5758R, 1:100, Bioss, Woburn, MA, USA), α-SMA (ab124964, 1:100, Abcam, Cambridge, MA, USA), and EpCAM (ab71916, 1:100, Proteintech, Chicago, IL, USA). .. Subsequently, they were treated with goat anti-rabbit secondary antibody (ABclonal, Wuhan, China) at 37 °C for 1 h. The cell nuclei were counterstained with DAPI (Solarbio, Beijing, China).

    Article Title: Modulation of Cancer-Associated Fibroblasts via the miR-624-5p/FAP Axis Drives Progression and Metastasis in Non-Small Cell Lung Cancer.
    Article Snippet: .. Cells were fixed with 4% paraformaldehyde for 30 min, followed by PBS washing, permeabilization with 0.5% Triton X-100 (Solarbio, Beijing, China), and blocking with 10% BSA, and then incubated overnight at 4 ◦C with antibodies against FAP (Bs-5758R, 1:100, Bioss, Woburn, MA, USA), α-SMA (ab124964, 1:100, Abcam, Cambridge, MA, USA), and EpCAM (ab71916, 1:100, Proteintech, Chicago, IL, USA). .. Subsequently, they were treated with goat anti-rabbit secondary antibody (ABclonal, Wuhan, China) at 37 ◦C for 1 h. The cell nuclei were counterstained with DAPI (Solarbio, Beijing, China).

    Incubation:

    Article Title: Modulation of Cancer-Associated Fibroblasts via the miR-624-5p/FAP Axis Drives Progression and Metastasis in Non-Small Cell Lung Cancer
    Article Snippet: .. Cells were fixed with 4% paraformaldehyde for 30 min, followed by PBS washing, permeabilization with 0.5% Triton X-100 (Solarbio, Beijing, China), and blocking with 10% BSA, and then incubated overnight at 4 °C with antibodies against FAP (Bs-5758R, 1:100, Bioss, Woburn, MA, USA), α-SMA (ab124964, 1:100, Abcam, Cambridge, MA, USA), and EpCAM (ab71916, 1:100, Proteintech, Chicago, IL, USA). .. Subsequently, they were treated with goat anti-rabbit secondary antibody (ABclonal, Wuhan, China) at 37 °C for 1 h. The cell nuclei were counterstained with DAPI (Solarbio, Beijing, China).

    Article Title: Modulation of Cancer-Associated Fibroblasts via the miR-624-5p/FAP Axis Drives Progression and Metastasis in Non-Small Cell Lung Cancer.
    Article Snippet: .. Cells were fixed with 4% paraformaldehyde for 30 min, followed by PBS washing, permeabilization with 0.5% Triton X-100 (Solarbio, Beijing, China), and blocking with 10% BSA, and then incubated overnight at 4 ◦C with antibodies against FAP (Bs-5758R, 1:100, Bioss, Woburn, MA, USA), α-SMA (ab124964, 1:100, Abcam, Cambridge, MA, USA), and EpCAM (ab71916, 1:100, Proteintech, Chicago, IL, USA). .. Subsequently, they were treated with goat anti-rabbit secondary antibody (ABclonal, Wuhan, China) at 37 ◦C for 1 h. The cell nuclei were counterstained with DAPI (Solarbio, Beijing, China).



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    (a–c) Flow-cytometry workflow and representative plots for (a) NF alone, (b) NF co-cultured with RT112, and (c) NF co-cultured with T24 for 48 h. Gates show total cells (FSC-A vs SSC-A), singlets (FSC-H vs FSC-A), and the HBSF population identified by Alexa Fluor 488 negative (pre-labelled cancer with CellTracker Green). The frequency of gated HBSF within each condition is indicated. Right-hand histograms display activation-marker staining in gated HBSF <t>(αSMA/FAP;</t> Alexa Fluor 647 channel). (d) Quantification of mean fluorescence intensity (MFI) for αSMA and FAP in HBSF monoculture versus direct co-culture with RT112 or T24 (48 h). Both co-cultures significantly increased <t>fibroblast</t> activation marker expression, with the strongest induction observed in T24– HBSF. Bars show mean ± SD from n = 3.
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    Figure 8. Immunohistochemical evaluation of excised tumor tissues from TUBO tumor-bearing Balb/C mice. Brown signals correspond to <t>anti-FAP</t> staining; nuclei were counterstained with hematoxylin. Immunohistochemical staining of excised tumor tissue in the absence of primary antibody anti-FAP above and immunohistochemical staining of excised tumor tissue (anti-FAP primary antibody) below.
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    Image Search Results


    (a–c) Flow-cytometry workflow and representative plots for (a) NF alone, (b) NF co-cultured with RT112, and (c) NF co-cultured with T24 for 48 h. Gates show total cells (FSC-A vs SSC-A), singlets (FSC-H vs FSC-A), and the HBSF population identified by Alexa Fluor 488 negative (pre-labelled cancer with CellTracker Green). The frequency of gated HBSF within each condition is indicated. Right-hand histograms display activation-marker staining in gated HBSF (αSMA/FAP; Alexa Fluor 647 channel). (d) Quantification of mean fluorescence intensity (MFI) for αSMA and FAP in HBSF monoculture versus direct co-culture with RT112 or T24 (48 h). Both co-cultures significantly increased fibroblast activation marker expression, with the strongest induction observed in T24– HBSF. Bars show mean ± SD from n = 3.

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    Article Title: Bidirectional Crosstalk Between Bladder Cancer Cells and Normal Fibroblasts Drives Phenotypic Reprogramming and Modulates Chemosensitivity

    doi: 10.1101/2025.09.09.675061

    Figure Lengend Snippet: (a–c) Flow-cytometry workflow and representative plots for (a) NF alone, (b) NF co-cultured with RT112, and (c) NF co-cultured with T24 for 48 h. Gates show total cells (FSC-A vs SSC-A), singlets (FSC-H vs FSC-A), and the HBSF population identified by Alexa Fluor 488 negative (pre-labelled cancer with CellTracker Green). The frequency of gated HBSF within each condition is indicated. Right-hand histograms display activation-marker staining in gated HBSF (αSMA/FAP; Alexa Fluor 647 channel). (d) Quantification of mean fluorescence intensity (MFI) for αSMA and FAP in HBSF monoculture versus direct co-culture with RT112 or T24 (48 h). Both co-cultures significantly increased fibroblast activation marker expression, with the strongest induction observed in T24– HBSF. Bars show mean ± SD from n = 3.

    Article Snippet: For NF, primary antibodies against fibroblast activation protein (FAP) and α-smooth muscle actin (αSMA) (Cell Signaling Technology) were applied at 1:100 overnight at 4°C.

    Techniques: Flow Cytometry, Cell Culture, Activation Assay, Marker, Staining, Fluorescence, Co-Culture Assay, Expressing

    Figure 8. Immunohistochemical evaluation of excised tumor tissues from TUBO tumor-bearing Balb/C mice. Brown signals correspond to anti-FAP staining; nuclei were counterstained with hematoxylin. Immunohistochemical staining of excised tumor tissue in the absence of primary antibody anti-FAP above and immunohistochemical staining of excised tumor tissue (anti-FAP primary antibody) below.

    Journal: Molecular pharmaceutics

    Article Title: Novel FAP-Targeted Heptamethine Cyanines for NIRF Imaging Applications.

    doi: 10.1021/acs.molpharmaceut.4c01232

    Figure Lengend Snippet: Figure 8. Immunohistochemical evaluation of excised tumor tissues from TUBO tumor-bearing Balb/C mice. Brown signals correspond to anti-FAP staining; nuclei were counterstained with hematoxylin. Immunohistochemical staining of excised tumor tissue in the absence of primary antibody anti-FAP above and immunohistochemical staining of excised tumor tissue (anti-FAP primary antibody) below.

    Article Snippet: FAP expression was measured by Western Blot using antibodies against FAP (Santa Cruz, sc-100528) and β actin as a loading control (Santa Cruz, sc-4778).

    Techniques: Immunohistochemical staining, Staining